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KMID : 1149220200280010081
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Korean Journal of Oriental Medical Prescription
2020 Volume.28 No. 1 p.81 ~ p.90
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Icariin promotes melanin synthesis
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Cha Su-Bin
Park Seol-A
Kang Lea Min-Ju
Woo Won-Hong
Mun Yeun-Ja
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Abstract
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Objectives : This study was conducted to investigate the effects of major constituents of Epimedium koreanum Nakai (Icariin, epimedium A, epimedium B, and epimedium C) on melanin synthesis.
Methods : We measured melanin contents, tyrosinase activity, and expression of Rab27a in B16F10 cells cultured with Epimedium koreanum Nakai ethanol extract (EKN) and their major constituents. After treatment with H89 and dibutyryl cAMP, which inhibit or promote the activation of PKA, we observed changes in melanin synthesis and tyrosinase activity stimulated by EKN.
Results : Among them, EKN and icariin enhanced tyrosinase activity and melanin contents. We confirmed that EKN augmented melanin synthesis via cAMP/PKA pathway. Icariin-induced tyrosinase activity and melanin content were attenuated by PKA inhibitor H89, while melanogenic effect of icariin was further augmented by cAMP analog, dbc AMP. However, icariin did not affect the expression of small GTPase Rab27a involved in melanosome transport.
Conclusions : These results suggest that icariin promotes melanogenesis through cAMP/PKA pathway but does not affect small GTPase Rab27a.
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KEYWORD
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icariin, Epimedium koreanum Nakai, melanin, tyrosinase, PKA, Rab27a
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